Association between prognostic survival of human colorectal carcinoma and ZNRF3 expression

abstract: ...Purpose E3 ubiquitin ligase ZNRF3 is linked to the pathogenesis of diseases and tumorigenesis. The present study aims to explore the expression of ZNRF3 and its association with prognostic survival of human colorectal carcinoma. Methods A follow-up survey of 168 patients with colorectal carcinoma was performed, and specimens of colorectal tissues were collected for immunohistochemistry and Western blotting analyses. Furthermore, overexpression of ZNRF3 using transient transfection with the recombinant pEGFP-ZNRF3 plasmid and detection of apoptosis and proliferation were performed in HCT-116 cells. Results The results showed a diverse feature of ZNRF3 staining, such as strong, moderate, weak, or negative, in colorectal carcinoma tissues. Interestingly, univariate KaplanMeier analysis showed that cases with strong or moderate expression of ZNRF3 showed an optimistic disease-free survival and overall survival compared with negative expression of ZNRF3, and multivariate Cox model demonstrated ZNRF3 as an independent prediction index for overall survival and disease-free survival. In vitro, the overexpression of ZNRF3 was related to the negative regulation of Wnt/-catenin pathway and referred to an induction of apoptosis and suppression of proliferation in ZNRF3-transfected HCT-116 cells. Conclusion Our results suggest that the higher expression of ZNRF3 acts as a novel marker of indicating the optimistic prognosis of colorectal carcinoma by suppressing cancer cell growth and facilitating apoptosis.

...Onco Targets Ther Onco Targets Ther OncoTargets and Therapy OncoTargets and therapy 1178-6930 Dove Medical Press 27822070 5094639 10.2147/OTT.S108134 ott-9-6679 Original Research Association between prognostic survival of human colorectal carcinoma and ZNRF3 expression Yu Nanhui 1 2 Zhu Hong 1 Tao Yiming 1 Huang Yongpan 3 Song Xiangping 1 Zhou Yuan 1 Li Yuqiang 1 Pei Qian 1 Tan Qiurong 2 Pei Haiping 1 1 Department of Gastrointestinal Surgery, Xiangya Hospital, Central South University 2

... this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution Non Commercial (unported, v3.0) License ( http://creativecommons.org/licenses/by-nc/3.0/ ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Purpose E3 ubiquitin ligase ZNRF3 is linked to the pathogenesis of diseases and tumorigenesis. The present study aims to explore the expression of ZNRF3 and its association with prognostic survival of human colorectal carcinoma. Methods A follow-up survey of 168 patients with colorectal carcinoma was performed, and specimens of colorectal tissues were collected for immunohistochemistry and Western blotting analyses. Furthermore, overexpression of ZNRF3 using transient transfection with the recombinant pEGFP-ZNRF3 plasmid and detection of apoptosis and proliferation were performed in HCT-116 cells. Results The results showed a diverse feature of ZNRF3 staining, such as strong, moderate, weak, or negative, in colorectal carcinoma tissues. Interestingly, univariate KaplanMeier analysis showed that cases with strong or moderate expression of ZNRF3 showed an optimistic disease-free survival and overall survival compared with negative expression of ZNRF3, and multivariate Cox model demonstrated ZNRF3 as an independent prediction index for overall survival and disease-free survival. In vitro, the overexpression of ZNRF3 was related to the negative regulation of Wnt/-catenin pathway and referred to an induction of apoptosis and suppression of proliferation in ZNRF3-transfected HCT-116 cells. Conclusion Our results suggest that the higher expression of ZNRF3 acts as a novel marker of indicating the optimistic prognosis of colorectal carcinoma by suppressing cancer cell growth and facilitating apoptosis. Keywords colorectal cancer prognostic survival E3 ubiquitin ligase ZNRF3

... homeostasis throughout development and into adulthood. At the same time, aberrant Wnt signaling underlies most of the pathological pathways and can significantly affect proliferation and apoptosis in human cancer. 8 However, effective Wnt inhibitors have not been developed, and even if they did exist, they would not inhibit Wnt signaling in tumors with certain pathway mutations. ZNRF3, a unique transmembrane E3 ubiquitin ligase, suppresses -catenin signaling initiated by endogenous Wnt proteins, which distinguishes ZNRF3 from other negative regulators of Wnt signaling, such as APC, AXIN, and GSK3 a/b. 9 The expression and function of ZNRF3 have been examined in gastric adenocarcinoma, and the mechanisms through which it suppresses proliferation and induces apoptosis are well understood. 8 However, the characteristics of ZNRF3 expression and their association with colorectal carcinoma remain unclear. Here, we aimed to investigate the profile of prognostic survival in patients with colorectal carcinoma. In

infinome is an open experiment to study human longevity. Link your 23andMe account for personalized reports.

Gene: ZNRF3 - zinc and ring finger 3 - (NCBI)

Images from Open Access Papers

Search Results from Open Access Papers