Prognostic significance of Traf2- and Nck- interacting kinase (TNIK) in colorectal cancer

abstract: ...Background The potential of expression profiling using microarray analysis as a tool to predict the prognosis for different types of cancer has been realized. This study aimed to identify a novel biomarker for colorectal cancer (CRC). Methods The expression profiles of cancer cells in 152 patients with stage I-III CRC were examined using microarray analysis. High expression in CRC cells, especially in patients with distant recurrences, was a prerequisite to select candidate genes. Thus, we identified seventeen candidate genes, and selected Traf2- and Nck-interacting kinase ( TNIK ), which was known to be associated with progression in CRC through Wnt signaling pathways. We analyzed the protein expression of TNIK using immunohistochemistry (IHC) and investigated the relationship between protein expression and patient characteristics in 220 stage I-III CRC patients. Results Relapse-free survival was significantly worse in the TNIK high expression group than in the TNIK low expression group in stage II ( p =0.028) and stage III ( p =0.006) patients. In multivariate analysis, high TNIK expression was identified as a significant independent risk factor of distant recurrence in stage III patients. Conclusion This study is the first to demonstrate the prognostic significance of intratumoral TNIK protein expression in clinical tissue samples of CRC, in that high expression of TNIK protein in primary tumors was associated with distant recurrence in stage II and III CRC patients. This TNIK IHC study might contribute to practical decision-making in the treatment of these patients. Electronic supplementary material The online version of this article (doi:10.1186/s12885-015-1783-y) contains supplementary material, which is available to authorized users.

...BMC Cancer BMC Cancer BMC Cancer 1471-2407 BioMed Central London 26499327 4619995 1783 10.1186/s12885-015-1783-y Research Article Prognostic significance of Traf2- and Nck- interacting kinase (TNIK) in colorectal cancer Takahashi Hidenori htsrg2@tmd.ac.jp 1 Ishikawa Toshiaki +81-3-5803-5261 ishi.srg2@tmd.ac.jp 1 Ishiguro Megumi ishiguro.srg2@tmd.ac.jp 2 Okazaki Satoshi okazaki3332@gmail.com 1 Mogushi Kaoru kmogushi@juntendo.ac.jp 3 Kobayashi Hirotoshi h-kobayashi.srg2@tmd.ac.jp 4 Iida Satoru s

... select candidate genes. Thus, we identified seventeen candidate genes, and selected Traf2- and Nck-interacting kinase ( TNIK ), which was known to be associated with progression in CRC through Wnt signaling pathways. We analyzed the protein expression of TNIK using immunohistochemistry (IHC) and investigated the relationship between protein expression and patient characteristics in 220 stage I-III CRC patients. Results Relapse-free survival was significantly worse in the TNIK high expression group than in the TNIK low expression group in stage II ( p =0.028) and stage III ( p =0.006) patients. In multivariate analysis, high TNIK expression was identified as a significant independent risk factor of distant recurrence in stage III patients. Conclusion This study is the first to demonstrate the prognostic significance of intratumoral TNIK protein expression in clinical tissue samples of CRC, in that high expression of TNIK protein in primary tumors was associated with distant recurrence in stage II and III CRC patients. This TNIK IHC study might contribute to practical decision-making in the treatment of these patients. Electronic supplementary material The online version of this article (doi:10.1186/s12885-015-1783-y) contains supplementary material, which is available to authorized users. Keywords Colorectal cancer Biomarker Microarray analysis Traf2- and Nck- interacting kinase ( TNIK ) Prognostic factor issue-copyright-statement The Author(s) 2015 Background Colorectal cancer (CRC

... Nck-interacting kinase ( TNIK ) using microarray analysis as a candidate gene that was related to distant recurrence of CRC. TNIK is a member of the germinal center kinase family, and is reported to be essential for Wnt signaling and CRC proliferation and progression [ 10 , 11 ]. However, the correlation between intratumoral TNIK expression and the prognosis of CRC patients has not yet been reported. This study aimed to confirm the prognostic significance of TNIK in CRC through investigation of the relationship between intratumoral TNIK protein expression and the prognosis of CRC patients. Methods Patients Primary tumors from 1210 consecutive patients who underwent surgical resection for CRC between 2002 and 2009 at Tokyo Medical and Dental University Hospital were included in this study. This study was conducted in accordance with the Declaration of Helsinki and Ethical Guidelines for Epidemiological Study published by the Japanese government. The Ethical Committee of Tokyo Medical and

... non-cancerous mucosa, (ii) a significantly higher expression level in cancer cells of the recurrence group than in the non-recurrence group. A higher expression was defined as a numerical value 1.5 times greater than those in another group. Immunohistochemistry A streptavidin-biotin method was used for TNIK immunostaining. Formalin-fixed paraffin-embedded tissue blocks from each patient were cut at 4-m thickness. After deparaffinization in xylene and rehydration through a series of incubations in

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Gene: TNIK - TRAF2 and NCK interacting kinase - (NCBI)

Protein : 2x7f

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