JMY Functions as Actin Nucleation-Promoting Factor and Mediator for p53-Mediated DNA Damage in Porcine Oocytes

abstract: ...Junction-mediating and regulatory protein(JMY) is a multifunctional protein with roles in the transcriptional co-activation of p53 and the regulation of actin nucleation promoting factors and, hence, cell migration; however, its role in the maturation of porcine oocytes is unclear. In the current study, we investigated functional roles of JMY in porcine oocytes. Porcine oocytes expressed JMY mRNA and protein, and the mRNA expression level decreased during oocyte maturation. Knockdown of JMY by RNA interference decreased the rate of polar body extrusion, validating its role in the asymmetric division of porcine oocytes. JMY knockdown also down-regulated the mRNA and protein levels of actin and Arp2/3. Furthermore, JMY accumulated in the nucleus in response to DNA damage, and JMY knockdown suppressed DNA damage-mediated p53 activation. In conclusion, our results show that JMY has important roles in oocyte maturation as a regulator of actin nucleation-promoting factors and an activator of p53 during DNA damage during DNA damages in porcine oocytes.

...PLoS One PLoS ONE plos plosone PLoS ONE 1932-6203 Public Library of Science San Francisco, USA 25279558 4184845 PONE-D-14-28546 10.1371/journal.pone.0109385 Research Article Biology and life sciences Biochemistry DNA DNA damage Cell Biology Cell Motility Actin Filaments Dynamic Actin Filaments Cellular Types Animal Cells Germ Cells OVA Oocytes JMY Functions as Actin Nucleation-Promoting Factor and Mediator for p53-Mediated DNA Damage in Porcine Oocytes JMY Regulates Actin and DNA Damage in

.../materials/analysis tools: NHK. Contributed to the writing of the manuscript: ZL SN NHK. 2014 3 10 2014 9 10 e109385 26 6 2014 10 9 2014 2014 Lin et al This is an open-access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Junction-mediating and regulatory protein(JMY) is a multifunctional protein with roles in the transcriptional co-activation of p53 and the regulation of actin nucleation promoting factors and, hence, cell migration; however, its role in the maturation of porcine oocytes is unclear. In the current study, we investigated functional roles of JMY in porcine oocytes. Porcine oocytes expressed JMY mRNA and protein, and the mRNA expression level decreased during oocyte maturation. Knockdown of JMY by RNA interference decreased the rate of polar body extrusion, validating its role in the asymmetric division of porcine oocytes. JMY knockdown also down-regulated the mRNA and protein levels of actin and Arp2/3. Furthermore, JMY accumulated in the nucleus in response to DNA damage, and JMY knockdown suppressed DNA damage-mediated p53 activation. In conclusion, our results show that JMY has important roles in oocyte maturation as a regulator of actin nucleation-promoting factors and an activator of p53 during DNA damage during DNA damages in porcine oocytes. This study was supported by a grant from the Next Generation

... signaling [3] , are also important for oocyte maturation. For example, Junction-mediating and regulatory protein (JMY), one of actinnucleation-promoting factor [8] , [9] is critical for spindle migration and asymmetric division in mouse oocytes [10] . In addition to its role as an actin nucleation promoting factor, JMY was originally identified as a p53 coactivator, localizes to the nucleus during DNA damage [11] [13] . Upon processing of DNA double-strand breaks (DSBs), JMY forms a complex with strap (stress-responsive activator of p300) and p300, which recruits PRMT5 into a coactivator complex that drives the p53 response [14] , [15] It is reported that DNA damage affects JMY activity, which is required for p53 transcriptional activity in MCF-7 cells [15] . Recent studies identify JMY as a target through which Mdm2 regulates p53 activity [12] . Considering dual roles of JMY as DNA damage responsive element and actin nucleation promoting factors in somatic cells, we postulated that JMY could be

... and oocytes. In the current study, we investigated the roles of JMY as a regulator of actin nucleation-promoting activity and also as an activator of p53 during DNA damage in maturing porcine oocytes. Materials and Methods In vitro maturation (IVM) of porcine oocytes Porcine ovaries were collected from gilts at a commercial slaughterhouse and transported to the laboratory in saline maintained at 37C. Cumulus oocyte complexes (COCs) were aspirated from follicles 26 mm in diameter using an 18-gauge

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Gene: JMY - junction mediating and regulatory protein, p53 cofactor - (NCBI)

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