Discovery of piRNAs Pathway Associated with Early-Stage Spermatogenesis in Chicken

abstract: ...Piwi-interacting RNAs (piRNAs) play a key role in spermatogenesis. Here, we describe the piRNAs profiling of primordial germ cells (PGCs), spermatogonial stem cells (SSCs), and the spermatogonium (Sp) during early-stage spermatogenesis in chicken. We obtained 31,361,989 reads from PGCs, 31,757,666 reads from SSCs, and 46,448,327 reads from Sp cells. The length distribution of piRNAs in the three samples showed peaks at 33 nt. The resulting genes were subsequently annotated against the Gene Ontology (GO) database. Five genes ( RPL7A , HSPA8 , Pum1 , CPXM2 , and PRKCA ) were found to be involved in cellular processes. Interactive pathway analysis (IPA) further revealed three important pathways in early-stage spermatogenesis including the FGF, Wnt, and EGF receptor signaling pathways. The gene Pum1 was found to promote germline stem cell proliferation, but it also plays a role in spermatogenesis. In conclusion, we revealed characteristics of piRNAs during early spermatogonial development in chicken and provided the basis for future research.

... primordial germ cells (PGCs), spermatogonial stem cells (SSCs), and the spermatogonium (Sp) during early-stage spermatogenesis in chicken. We obtained 31,361,989 reads from PGCs, 31,757,666 reads from SSCs, and 46,448,327 reads from Sp cells. The length distribution of piRNAs in the three samples showed peaks at 33 nt. The resulting genes were subsequently annotated against the Gene Ontology (GO) database. Five genes ( RPL7A , HSPA8 , Pum1 , CPXM2 , and PRKCA ) were found to be involved in cellular

... analyses We mapped the 153 validated piRNAs sequences onto the chicken reference genome ( Gallus gallus v.4) using Bowtie 2.0. After filtering for repeats, 22 genes were annotated ( S3 Table ). Gene Ontology (GO) analysis of these genes showed that nearly 75% of the genes are related to cellular processes (including RPL7A , PRKCA , PUM1 , HSPA8 , and CPXM2 ) while 20% of the genes are involved in developmental processes within the Biological Process category. Within the Cellular Component category

... within the Biological Process category, nearly 60% of genes were related to cellular processes (including RPL7A , PUM1 , CPXM2) , while 20% of genes were involved in cellular component biogenesis. Nearly 75% of genes are present in the cell part within the Cellular Component category, and 55% of genes were associated with binding, including ATP binding, nucleotide binding, or metal ion binding among the Molecular Function category ( Fig 9 ). In the KEGG analysis, genes were mainly identified in two

... spermatogenesis We searched for candidate genes and pathways with two separate approaches. First, we identified genes with piRNAs common to all three cell types. Using this approach, we identified 22 genes that are involved in processes such as immune system processes, death, pigmentation, and biological regulation. We also acquired 5 genes involved in cellular processes ( RPL7A , HSPA8 , Pum1 , CPXM2 , and PRKCA) . Protein kinase C alpha ( PRKCA ) is a key element with many functions, including the ability

... protein folding, and ribosomal Protein L7a ( RPL7A ) mainly participates in ribosome biogenesis according to the GO analysis. Carboxypeptidase X (M14 family) member 2 ( CPXM2 ) is related to cell adhesion according to the GO analysis. Using the second approach of identifying the common different piRNAs, results showed that RPL7A , Pum1 , and CPXM2 were involved in this process. These genes overlap with the genes from the former approach. According to KEGG analysis, 3 out of 26 pathways were related to

... difference between Pum1 , HSPA8 , PRKCA , and RPL7A genes in PGCs and SSCs but in Sp there was significant difference in these genes. The PRKCA gene were expressed highest in Sp and lowest in PGCs. Furthermore, except PRKCA, the other three genes expressed higher in PGCs and SSCs than in Sp, indicating that they play a role in the three cell types. On the other hand, CPXM2 expression showed significant differences in the three cell types. Notably, the expression of PRKCA in Sp was much higher than in the

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Gene: CPXM2 - carboxypeptidase X (M14 family), member 2 - (NCBI)

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