Expression of CPEB4 in Human Glioma and Its Correlations With Prognosis

abstract: ...Abstract CPEB4 plays an important role in cancer progression. However, the clinicopathological significance of CPEB4 expression to glioma and its expression levels in glioma tissues and cell lines are unknown. The present study investigated the potential prognostic value of CPEB4 for human glioma. Immunohistochemistry (IHC) was performed to examine the dynamics of CPEB4 expression in glioma and nonneoplastic brain tissues, and the expression of CPEB4 in cell lines and freshly prepared tissue samples was measured using Western blotting and real-time PCR. CPEB4 was highly expressed at the mRNA and protein levels in 4 glioma cell lines and in 4 freshly prepared glioma tissues. Immunohistochemical analysis demonstrated that CPEB4 expression in glioma tissue was higher than that in corresponding nonneoplastic brain tissue ( P <0.01). This high expression level was further increased in high-grade gliomas, and the CPEB4 expression level correlated with the WHO classification (r=0.774, P <0.01). Moreover, the overall survival of glioma patients displaying high CPEB4 protein expression ( P <0.01) was clearly lower than that of those displaying low CPEB4 expression, and the high CPEB4 expression indicated a poorer survival in high-grade glioma patients ( P <0.01). Our study suggests that CPEB4 is significantly expressed in human glioma and that the upregulation of CPEB4 protein is significantly associated with advanced WHO grade. CPEB4 may serve as a highly sensitive prognostic indicator for glioma patients.

...Medicine (Baltimore) Medicine (Baltimore) MEDI Medicine 0025-7974 1536-5964 Lippincott Williams & Wilkins 26166131 4504610 10.1097/MD.0000000000000979 00979 5700 Research Article Observational Study Expression of CPEB4 in Human Glioma and Its Correlations With Prognosis Hu Wanming MM Yang Yuanzhong MB Xi Shaoyan MM Sai Ke MD, PhD Su Dongfang PhD Zhang Xinke MM Lin Suxia MD, PhD Zeng Jing MD, PhD Li. Bo From Department of Pathology, Cancer Center, Sun Yat-Sen University, Guangzhou, China (WH, YY

... Yat-Sen University, Cancer Center, Guangzhou, Guangdong, China (e-mail: zengjing1320@163.com ). 7 2015 13 7 2015 94 27 e979 24 10 2014 10 5 2015 18 5 2015 Copyright 2015 Wolters Kluwer Health, Inc. All rights reserved. 2015 This is an open access article distributed under the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0 Abstract CPEB4 plays an important role in cancer progression. However, the clinicopathological significance of CPEB4 expression to glioma and its expression levels in glioma tissues and cell lines are unknown. The present study investigated the potential prognostic value of CPEB4 for human glioma. Immunohistochemistry (IHC) was performed to examine the dynamics of CPEB4 expression in glioma and nonneoplastic brain tissues, and the expression of CPEB4 in cell lines and freshly prepared tissue samples was measured using Western blotting and real-time PCR. CPEB4 was highly expressed at the mRNA and protein levels in 4 glioma cell lines and in 4 freshly prepared glioma tissues. Immunohistochemical analysis demonstrated that CPEB4 expression in glioma tissue was higher than that in corresponding nonneoplastic brain tissue ( P <0.01). This high expression level was further increased in high-grade gliomas, and the CPEB4 expression level correlated with the WHO classification (r=0.774, P <0.01). Moreover, the overall survival of glioma patients displaying high CPEB4 protein expression ( P <0.01) was clearly lower than that of those displaying low CPEB4 expression, and the high CPEB4 expression indicated a poorer survival in high-grade glioma patients ( P <0.01). Our study suggests that CPEB4 is significantly expressed in human glioma and that the upregulation of CPEB4 protein is significantly associated with advanced WHO grade. CPEB4 may serve as a highly sensitive prognostic indicator for glioma patients

... diagnosis. 4 Nonetheless, as the prognosis of gliomas remains heterogeneous, the identification of diagnostic and prognostic markers of glioma is urgently needed to devise more effective individually targeted therapies for this disease. Cytoplasmic polyadenylation element binding protein 4 (CPEB4) is a sequence-specific RNA-binding protein that participates in translational control. CPEB4 is a member of the CPEB family, which includes CPEB1, CPEB2, CPEB3, and CPEB4, all of which share structure and sequence identity in the C-terminal RNA-binding domain (RBD). CPEB2, CPEB3, and CPEB4 are more closely related to each other than to CPEB1. For example, CPEB2 to 4 exhibit 96% and 2535% sequence identity in the RBD and N-terminal regulatory regions, respectively, but they share only 45% sequence identity with CPEB1 in the RBD region. 5 Recent studies suggest that CPEB4 regulates a wide range of biological processes involved in tumor and progression, such as tumor cell growth, vascularization, invasion

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Gene: CPEB4 - cytoplasmic polyadenylation element binding protein 4 - (NCBI)

Protein : 2mkj

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